EXPRESSION OF HUMAN PLASMINOGEN-ACTIVATOR INHIBITOR-2 IN THE BACULOVIRUS EXPRESSION SYSTEM

Authors
PEI Y ZHOU AW CAO XR XU XX
Citation
Y. Pei et al., EXPRESSION OF HUMAN PLASMINOGEN-ACTIVATOR INHIBITOR-2 IN THE BACULOVIRUS EXPRESSION SYSTEM, Biochemistry and molecular biology international, 35(5), 1995, pp. 923-928
Citations number
24
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
35
Issue
5
Year of publication
1995
Pages
923 - 928
Database
ISI
SICI code
1039-9712(1995)35:5<923:EOHPII>2.0.ZU;2-Q
Abstract
A cDNA coding for human plasminogen activator inhibitor-2 (PAI-2) was transfered into the genome of Autographa californica nuclear polyhedro sis virus adjacent to the polyhedrin promoter. Cells infected with rec ombinant virus synthesized a 43-kDa PAI-2 protein, the majority of whi ch was secreted. The recombinant PAI-2 was identical to native PAI-2 p urified from human placenta with respect to interaction with polyclona l antibody and inhibition of urokinase-type plasminogen activator.