COMMON PROMOTER FEATURES IN HUMAN AND MOUSE-LIVER TYPE PHOSPHOFRUCTOKINASE GENE

The liver-type phosphofructokinase is a key glycolytic enzyme encoded by genes residing on human and mouse chromosomes 21 and 10 respectivel y. Genomic DNA regions upstream of the initiator ATG spanning 2.6 Kb a nd 3.4 Kb of human and mouse liver-type phosphofructokinase gene were sequenced and analyzed. The proximal 0.4 Kb region of both genes featu red a CpG island containing 60%-73% GC residues. The first 120 nucleot ides preceding the ATG are highly conserved displaying 73% of sequence similarity between human and mouse genes. While this region lacks TAT A and GAAT boxes it contains four Spl binding sites and was capable of promoting a non regulated expression of the reporter gene chloramphen icol acetyl transferase, in transfection assays. Additional conserved elements were found further upstream at the 5'-region of both the huma n and mouse genes. They consisted of two Alu repeats and several seque nce motifs known to serve as transcription factors binding sites.