D. Klein et al., A METHOD FOR QUANTIFICATION AND CORRECTION OF PROTEINS AFTER TRANSFERTO IMMOBILIZATION MEMBRANES, Biochemistry and molecular biology international, 36(1), 1995, pp. 59-66
A method is described for quantification of specific proteins after el
ectrophoresis and transfer to immobilization membranes for Western blo
ts. This method is analogous to methods used to correct the amounts of
specific transcripts detected on Northern blots. Ponceau S staining o
f proteins bound to immobilization membranes is efficient and accurate
compared to antibody binding in terms of time, effort and cost. Compa
rison of Ponceau S to other detection and staining methods for quantif
ying proteins and the basic chemistry of Ponceau S are summarized.