A NEW METHOD OF HUMAN CARDIAC TROPONIN-I AND TROPONIN-T PURIFICATION

New rapid and effective procedure for simultaneous purification of hum an cardiac troponin I and troponin T has been developed. Affinity chro matography on immobilized monoclonal antitroponin I antibody C5 was us ed for purification of the whole troponin complex with a yield of 120 mg from 100 g of tissue. Isolated troponin I and troponin T (about 20 and 35 mg from 100 g tissue) were obtained by conventionally used ion- exchange chromatography. Antibody C5 recognizes conservative epitope o f troponin I, therefore the method is applicable for purification of s keletal and cardiac troponin from a number of different animal species .