Eg. Elias et al., ADJUVANT IMMUNOTHERAPY IN MELANOMA WITH IRRADIATED AUTOLOGOUS TUMOR-CELLS AND LOW-DOSE CYCLOPHOSPHAMIDE, Journal of surgical oncology, 64(1), 1997, pp. 17-22
Background: Patients with metastatic melanoma to their regional lymph
nodes have a poor prognosis despite lymphadenectomy. In an attempt to
improve their survival, this feasibility study was undertaken. Methods
: Twenty-two melanoma patients, who presented with enlarged regional l
ymph nodes, underwent therapeutic lymphadenectomy. They were found to
have N-2 nodal disease, with no evidence of distant metastases, i.e.,
advanced Stage III disease, One month after the lymphadenectomy, each
patient received five autologous tumor vaccines. Each vaccine consiste
d of 20 x 10(6) irradiated autologous tumor cells (20,000 cGy) injecte
d intradermally. The first two vaccines contained BCG and were given 1
week apart. The other three vaccines consisted of irradiated tumor ce
lls only without BCG, administered over 2-, 4-, and 8- week intervals,
respectively. Cyclophosphamide was administered intravenously as 300
mgm/m(2) 3 days prior to vaccines 1, 4, and 5 to reduce the population
of T-suppressor cells. The patients were observed with no additional
therapy. Three patients developed recurrences and these site were rese
cted, and the patients were revaccinated in the same fashion utilizing
the new tumor cells. Results: After a follow-up of 4-6 years, 15 pati
ents (including 3 who were revaccinated) of the initial 22 patients (6
8.2%) are alive free of disease. Conclusions: Adjuvant immunotherapy w
ith irradiated autologous melanoma cells and low dose cyclophosphamide
seemed to yield better relapse-free survival than the historically re
ported 10-25%. (C) 1997 Wiley-Liss, Inc.