A. Nagyova et E. Ginter, THE INFLUENCE OF ASCORBIC-ACID ON THE HEPATIC CYTOCHROME-P-450, AND GLUTATHIONE IN GUINEA-PIGS EXPOSED TO 2,4-DICHLOROPHENOL, Physiological Research, 44(5), 1995, pp. 301-305
Chlorophenols, mainly used as biocides, are compounds with a wide spec
trum of toxic effects including teratogenic and carcinogenic actions.
In this study, the effects of 2,4-dichlorophenol (2,4-DCP) on hepatic
microsomal cytochrome P-450, NADPH-cytochrome c reductase activity, li
ver ascorbic acid (AA) and glutathione (GSH) content were studied in g
uinea-pigs with a low (2 mg/day/animal) or a high (50 mg/day/animal) a
scorbic acid intake. The high AA intake significantly increased liver
AA and GSH levels. There was a clear-cut correlation between liver AA
and GSH levels. Administration of 2,4-DCP significantly decreased cyto
chrome P-450 and NADPH-cytochrome c reductase activity in hepatic micr
osomes isolated from guinea-pigs with the low AA intake. Such a reduct
ion was not observed in intoxicated guinea-pigs with the high AA intak
e. The results suggest that AA can play a protective role In 2,4-DCP t
oxicity.