THE EFFECT OF POSTASPHYXIAL REOXYGENATION WITH 21-PERCENT VS 100-PERCENT OXYGEN ON NA-ATPASE ACTIVITY IN STRIATUM OF NEWBORN PIGLETS(,K+)

To compare the effect of 21% vs. 100% oxygen during post-asphyxial reo xygenation on brain cell membrane function in the striatum, 20 anesthe tized, ventilated newborn piglets were studied: group 1 (normoxia, n = 5), group 2 (asphyxia, no reoxygenation, n = 5), group 3 (asphyxia fo llowed by reoxygenation with 21% O-2, n = 5), and group 4 (asphyxia fo llowed by reoxygenation with 100% O-2, n = 5). Asphyxia was induced by a stepwise reduction in FiO(2) at 20 min intervals from 21% to 14%, 1 1%, and 8%. Following a total 60 min of asphyxia, piglets in groups 3 and 4 were recovered for 2 h with either 21% or 100% O-2. Na+,K+-ATPas e activity (mu mol P-i/mg protein/h) in striatal cell membranes was 31 +/- 1, 22 +/- 2, 32 +/- 2 and 26 +/- 1 in groups 1, 2, 3 and 4, respe ctively. Na+,K+-ATPase activities in groups 2 and 4 were significantly lower than in groups 1 and 3 (p < 0.01). Piglets recovered post-asphy xia for 2 h with 21% O-2 had restoration of Na+,K+-ATPase activity to baseline levels, while those treated with 100% O-2 during recovery had persistent Na+,K+-ATPase inhibition of 16%. This could result from in creased free radical production during reoxygenation with 100% O-2 whi ch could contribute to post-asphyxial cellular injury in the striatum.