Pt. Odinot et al., PCR-BASED CHARACTERIZATION OF YERSINIA-ENTEROCOLITICA - COMPARISON WITH BIOTYPING AND SEROTYPING, Epidemiology and infection, 115(2), 1995, pp. 269-277
PCR-based DNA fingerprinting was used to characterize 48 clinical isol
ates of Yersinia enterocolitica. The samples were examined by random a
mplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-
PCR with two enterobacterial repetitive intergenic consensus primers r
esulted in patterns which were poorly discriminated; 2 of 11 arbitrary
primers (RAPD-PCR) provided sufficient discriminatory power. In compa
risons with serotyping and biotyping, RAPD-fingerprinting was the most
discriminatory technique and may therefore be a valuable epidemiologi
cal tool for the study of Y. enterocolitica infections.