PCR-BASED CHARACTERIZATION OF YERSINIA-ENTEROCOLITICA - COMPARISON WITH BIOTYPING AND SEROTYPING

Citation
Pt. Odinot et al., PCR-BASED CHARACTERIZATION OF YERSINIA-ENTEROCOLITICA - COMPARISON WITH BIOTYPING AND SEROTYPING, Epidemiology and infection, 115(2), 1995, pp. 269-277
Citations number
25
Categorie Soggetti
Infectious Diseases
Journal title
ISSN journal
09502688
Volume
115
Issue
2
Year of publication
1995
Pages
269 - 277
Database
ISI
SICI code
0950-2688(1995)115:2<269:PCOY-C>2.0.ZU;2-I
Abstract
PCR-based DNA fingerprinting was used to characterize 48 clinical isol ates of Yersinia enterocolitica. The samples were examined by random a mplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR- PCR with two enterobacterial repetitive intergenic consensus primers r esulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discriminatory power. In compa risons with serotyping and biotyping, RAPD-fingerprinting was the most discriminatory technique and may therefore be a valuable epidemiologi cal tool for the study of Y. enterocolitica infections.