DOG(R)1 AND DOG(R)2 - 2 GENES FROM SACCHAROMYCES-CEREVISIAE THAT CONFER 2-DEOXYGLUCOSE RESISTANCE WHEN OVEREXPRESSED

Saccharomyces cerevisiae contains two genes (DOG(R)1 and DOG(R)2) that are able to confer 2-deoxyglucose resistance when they are overexpres sed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2-deoxyglucose-6 phosphate ( 2-DOG-6P) phosphatase activity. These enzymes have been purified and c haracterized. Dog(R)1p shows an optimum pH of 6, an optimum temperatur e of 30 degrees C and a K-M on 2-DOG-6P of 17 mM. Dog(R)2p shows a sim ilar optimum pH, but the optimum temperature is 40 degrees C and it ex hibits a K-M on 2-DOG-6P of 41 mM. Both enzymes require 10 mM-MgCl2 fo r maximal activity and they are inhibited by inorganic phosphate.