DNA-SEQUENCE ANALYSIS OF A 13 KBP FRAGMENT OF THE LEFT ARM OF YEAST CHROMOSOME-XV CONTAINING 7 NEW OPEN READING FRAMES

The sequence of a 13 kbp fragment located in the vicinity of the left telomere of chromosome XV (cosmid pEOA179) has been determined. Seven new open reading frames (ORFs) encoding polypeptides longer than 100 r esidues have been found (AOB629, AOA342, AOC231, AOE555, AOE236, AOA23 6 and AOE1045). Three of them show no identity with proteins deposited in the data banks. ORF AOB629 (629 amino acids) has some similarity w ith previously described ferric reductases from Saccharomyces cerevisi ae and Schizosaccharomyces pombe. ORF AOA342 encodes a polypeptide rem iniscent of dihydroflavonol-4-reductases from a number of plant specie s. AOE236 displays a high level of identity when compared with peroxis omal membrane proteins previously cloned from the methylotrophic yeast Candida boidinii. Finally, AOE1045 encodes a large protein (1045 resi dues) with some identity with a hypothetical 147 kDa protein identifie d during the sequencing of Caenorhabditis elegans chromosome 3. The co mplete nucleotide sequence of the 13 kbp fragment has been deposited a t the EMBL data base (Accession Number Z48239).