SEQUENCE-ANALYSIS OF A 30 KB DNA SEGMENT FROM YEAST CHROMOSOME-XIV CARRYING A RIBOSOMAL-PROTEIN GENE-CLUSTER, THE GENES ENCODING A PLASMA-MEMBRANE PROTEIN AND A SUBUNIT OF REPLICATION FACTOR-C, AND A NOVEL PUTATIVE SERINE THREONINE PROTEIN-KINASE GENE

We have determined the nucleotide sequence of a 30 kb fragment of chro mosome XIV of Saccharomyces cerevisiae. The sequence revealed the pres ence of 19 open reading frames (oRFs) longer than 300 bp. NO422 and NO 425 corrrespond to the split ribosomal protein genes encoding S16A and rp28, respectively, NO450 displays a striking similarity with serine/ threonine protein kinase genes, in particular with STE20, and therefor e may encode a novel member of this protein family. NO453 is the longe st ORF in this DNA segment, having a size of 4908 bp, but its function is not yet known. NO530 encodes the plasma membrane protein Mid1p and NO533 corresponds to the gene coding for a 40 kDa subunit of replicat ion factor C. The remaining oRFs show weak or no homology with protein s in the data bases. The sequence has been submitted to the EMBL data library under Accession Number U23084.