INFLUENCE OF PENTAERYTHRITOL TRIACRYLATE ACRYLIC CARRIER SWELLING ON IMMOBILIZATION OF ENZYMES

Suspension copolymerization and modification of pentaerythritol triacr ylate (PENTA)-butyl acrylate (BA) matrices with ethylenediamine (EtDA) are discussed. It is shown that aminolysis in water solution offers g el structure carriers with small pore radii (about 3 nm). These carrie rs are characterized by the high swelling and sharp correlation betwee n the pH value and the specific volume. The enzymes (acylase, glucoamy lase, and peroxydase) are immobilized by means of the glutaraldehyde m ethod. The best matrix seems to be copolymers modified in a 95% soluti on of ethylenediamine in water. Preparations do not lose activity afte r 1 month storage at 4 degrees C. (C) 1995 John Wiley & Sons, Inc.