CRYSTALLIZATION OF THREONYL-TRANSFER-RNA SYNTHETASE FROM THERMUS-THERMOPHILUS AND PRELIMINARY CRYSTALLOGRAPHIC DATA

Threonyl-tRNA synthetase from Thermus thermophilus (ttTRS) has been ov erproduced in Escherichia coli, purified and crystallized in solutions containing ammonium sulfate and glycerol. The crystals grew in the or thorhombic space group C222(1) with unit cell dimensions a = 119.5 Ang strom, b = 120.0 Angstrom, c = 317.5 Angstrom. The asymmetric unit is constituted of two monomers and the crystals contain 66% solvent. This paper reports the first crystals of ttTRS and preliminary crystallogr aphic results since the presumed crystals of ttTRS described in a prev ious paper [1] were crystals of aspartyl-tRNA synthetase [2].