R. Selvam et M. Adhirai, VITAMIN-E PRETREATMENT PREVENTS CYCLOSPORINE-A-INDUCED CRYSTAL DEPOSITION IN HYPEROXALURIC RATS, Nephron, 75(1), 1997, pp. 77-81
The in vivo effect of cyclosporin A (CsA) on renal calcium oxalate (Ca
Ox) crystal retention in experimental hyperoxaluric rats was investiga
ted, Further, the effect of pretreatment of vitamin E on the above con
ditions was also studied, Male Wistar rats were divided into two major
groups each containing 40 rats, One of the groups was pretreated with
vitamin E, Both major groups were then subgrouped into four groups: g
roup 1 received the vehicle (olive oil); group 2 received CsA in olive
oil (50 mg/kg); group 3 received 3% ammonium oxalate (AmOx), and grou
p 4 received CsA + AmOx, Nephrotoxicity was assessed by the activities
of urinary marker enzymes and also by histopathology, Urinary oxalate
excretion as well as the activities of lactate dehydrogenase, gamma-g
lutamyltranspeptidase, alkaline phosphatase and inorganic pyrophosphat
ase enzymes were elevated either in CsA-alone or AmOx-alone treated gr
oups, On combined administration of both CsA and AmOx, further elevati
ons of these enzymes were observed, Urinary excretion of oxalate conce
ntration positively correlated with urinary excretion of these enzymes
, Deposition of CaOx crystals was seen only in the kidneys of rats tha
t received combined treatment, On pretreatment with vitamin E the obse
rved increased urinary activities of the enzymes and oxalate, histopat
hological changes and the deposition of CaOx crystals by administratio
n of CsA in hyperoxaluria were prevented suggesting that vitamin E cou
ld be supplemented to prevent CsA-induced membrane damage.