Gm. Bolwig et al., MOLECULAR-CLONING OF LINOTTE IN DROSOPHILA - A NOVEL GENE THAT FUNCTIONS IN ADULTS DURING ASSOCIATIVE LEARNING, Neuron, 15(4), 1995, pp. 829-842
The linotte (Iio) gene was identified in a screen for mutations that d
isrupted 3 hr memory after olfactory associative learning, without aff
ecting the perception of odors or electroshock. The mutagenesis yielde
d a transposon-tagged gene disruption, which allowed rapid cloning of
genomic DNA. The Iio transcription unit was identified via rescue of t
he Iio(1) learning/memory defect by induced expression of a Iio(+) tra
nsgene in adults. The perception of odors or electroshock remained nor
mal when the Iio(+) transgene was expressed in these Iio(1) flies. Lea
rning/memory remained normal when the Iio(+) transgene was expressed i
n wild-type (Iio(+)) flies. The Iio gene produces only one transcript,
the level of expression of which varies throughout development. Seque
nce analysis indicates that Iio encodes a novel protein.