PHYSICAL AND GENETIC MAP OF THE GENOME OF CAMPYLOBACTER-UPSALIENSIS

A physical map of Campylobacter upsaliensis ATCC 43954 was constructed from DNA fragments generated by Sa/I (5' G/TCGAC), NarI (5' GG/CGCC) and BssHII (5' G/CGCGC) restriction digests separated using pulsed-fie ld gel electrophoresis. The size of the C. upsaliensis genome was appr oximately 2000 kb, providing evidence of the largest Campylobacter gen ome sized to date. Twenty-one fragments created from these restriction digests were assembled into a physical map using a combination of com plementary methods including cross-Southern hybridization, hybridirati on fingerprint analysis and hybridization with homologous and heterolo gous (from Campylobacter ieiunr3 gene probes. The position of ten gene tic loci, including that of the iron-uptake regulatory (fur) gene, wer e localized to the physical map. A genomic library of C. upsaliensis A TCC 43954 was constructed in lambda Gem-11 vector. Fifty thousand reco mbinants with an average sire of 16 kb represent a library about 200 t imes the size of the genome. Using C. jejuni DNA probes, clones repres enting C. upsaliensis flaA, fur and ftsZ genes were isolated and local ized to the physical map.