MASS-SPECTROMETRIC ANALYSIS OF CHITIN OLIGOSACCHARIDES PRODUCED BY RHIZOBIUM NODC PROTEIN IN ESCHERICHIA-COLI

Authors
KAMST E VANDERDRIFT KMGM THOMASOATES JE LUGTENBERG BJJ SPAINK HP
Citation
E. Kamst et al., MASS-SPECTROMETRIC ANALYSIS OF CHITIN OLIGOSACCHARIDES PRODUCED BY RHIZOBIUM NODC PROTEIN IN ESCHERICHIA-COLI, Journal of bacteriology, 177(21), 1995, pp. 6282-6285
Citations number
17
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
177
Issue
21
Year of publication
1995
Pages
6282 - 6285
Database
ISI
SICI code
0021-9193(1995)177:21<6282:MAOCOP>2.0.ZU;2-Q
Abstract
A system for studying the in vivo activity of Rhizobium NodC protein i n Escherichia coli has been developed, Using thin-layer chromatography , high-performance liquid chromatography, and mass spectrometry, we sh ow that in this system R. leguminosarum by. viciae NodC protein direct s the synthesis of chitinpentaose, chitintetraose, chitintriose, and t wo as yet unidentified modified chitin oligosaccharides.