IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF ONE OF THE SPORE GERMINATION PROTEINS, GERAB, IN BACILLUS-SUBTILIS SPORES

Authors
SAKAE Y YASUDA Y TOCHIKUBO K
Citation
Y. Sakae et al., IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF ONE OF THE SPORE GERMINATION PROTEINS, GERAB, IN BACILLUS-SUBTILIS SPORES, Journal of bacteriology, 177(21), 1995, pp. 6294-6296
Citations number
18
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
177
Issue
21
Year of publication
1995
Pages
6294 - 6296
Database
ISI
SICI code
0021-9193(1995)177:21<6294:IMLOOO>2.0.ZU;2-X
Abstract
Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was inves tigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immnnoglobulin G complex. Immunogold particles were visualized in the boundary region between th e cortex and coat of dormant spores, and they were broadly dispersed i nto the cortex region after germination.