DETERMINATION OF COPPER IN HUMAN PLASMA BY STRIPPING POTENTIOMETRY ONA MERCURY FILM ELECTRODE IN ETHYLENEDIAMINE MEDIUM

The use of ethylenediamine improves the determination of copper by pot entiometric stripping analysis on a mercury film electrode, with respe ct to the usual determination method in acidic medium. The influence o f chemical and methodological parameters is discussed, and the reliabi lity of the method is checked with certified reference materials. Infl uence of the acid digestion protocol in open and high pressure closed vessels is investigated. The method developed has been applied to the measurement of the copper level in mineralized human plasma samples. T he results have been compared with those obtained by electrothermal at omic absorption spectroscopy in a graphite furnace.