Db. Gomis et al., DETERMINATION OF FAT-SOLUBLE VITAMINS BY CAPILLARY LIQUID-CHROMATOGRAPHY IN BOVINE BLOOD-PLASMA, Analytica chimica acta, 315(1-2), 1995, pp. 177-181
The fat-soluble vitamins retinyl palmitate, D-3, E and K-1 were separa
ted on a packed reversed-phase fused-silica capillary column using met
hanol-tetrahydrofuran as mobile phase, and determined with UV detectio
n. Conventional liquid chromatographic equipment was adapted for such
purposes, providing a highly efficient and sensitive analytical system
. The vitamins could be separated isocratically with detection limits
of 2.9 pg, 1 pg, 29 pg and 2.6 pg, respectively. Application of the pr
oposed method to the quantification of fat-soluble vitamins in blood i
s reported.