DETERMINATION OF FAT-SOLUBLE VITAMINS BY CAPILLARY LIQUID-CHROMATOGRAPHY IN BOVINE BLOOD-PLASMA

The fat-soluble vitamins retinyl palmitate, D-3, E and K-1 were separa ted on a packed reversed-phase fused-silica capillary column using met hanol-tetrahydrofuran as mobile phase, and determined with UV detectio n. Conventional liquid chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system . The vitamins could be separated isocratically with detection limits of 2.9 pg, 1 pg, 29 pg and 2.6 pg, respectively. Application of the pr oposed method to the quantification of fat-soluble vitamins in blood i s reported.