PHYSIO-BIOCHEMICAL REGULATION AND NOVEL PROTEIN-INDUCED SPECIFICALLY DURING C-P COMPOUND BIODEGRADATION IN PSEUDOMONAS SP STRAIN NUMBER-A1

In this study, a strain of bacteria was isolated from herbicide-treate d soil which efficiently utilizes the herbicide glyphosate (GPS; N-pho sphonomethylglycine), one of the phosphonates (Pn), as the sole phosph orus (P) source, The degrading pathway of glyphosate in the isolate wa s examined by a physiological and biochemical method, and was investig ated for a specific inducible protein for degrading glyphosate and oth er phosphonates such as methylphosphonate (MPn) and 2-aminoethylphosph onate (AEPn), The isolated GPS biodegrading bacteria from the soil wer e identified as Pseudomonas sp,, which was also able to use C-P compou nds, such as AEPn and MPn, as the sole P source, When GPS was supplied with inorganic phosphate at a low concentration, Pseudomonas sp, stra in #A1 revealed a diauxic pattern in which it preferred to utilize ino rganic phosphate rather than GPS as a sole P source, As a result of an analysis on the degradation products of GPS by thin layer chromatogra phy, it showed that GPS degradation occurred via the C-P lyase pathway where the sarcosine was produced by a direct cut of C-P bond as a pri mary metabolite and transformed into glycine, Protein patterns were co mpared between the case of Pi starvation and that of some phosphonates , including GPS, As a result, two specific protein bands of molecular weight 62,000 and 132,000 were additionally detected, which seemed to be a porin protein specifically induced by the C-P compound or gene pr oducts related to the C-P lyase pathway or phosphonatase pathway.