SALT-DEPENDENT AND PH-DEPENDENT HEMAGGLUTINATION WITH KASBA-VIRUS, A MEMBER OF THE PALYAM SEROGROUP OF THE GENUS ORBIVIRUS

Kasba virus grown in BHK21-WI2 cells was tested for hemagglutination ( HA) with erythrocytes of a variety of species at 4 degrees C, 25 degre es C and 37 degrees C. HA was observed at all temperatures with cattle , sheep and goat but not with swine, chicken, and goose erythrocytes. The HA was dependent on not only the NaCl concentration but also the p H of the diluent. The HA titer significantly improved by increasing th e NaCl molarity to 0.6 M and standardizing pH to 7.5. The HA titer was 16- or 32-fold higher in 0.4 M or 0.6 M solution than in 0.2 M soluti on of not only NaCl but also several other salts. The HA reaction was inhibited by specific antibody.