CONSTRUCTION OF A RECOMBINANT CELLULOLYTIC ESCHERICHIA-COLI

Authors
SRIVASTAVA R KUMAR GP SRIVASTAVA KK
Citation
R. Srivastava et al., CONSTRUCTION OF A RECOMBINANT CELLULOLYTIC ESCHERICHIA-COLI, Gene, 164(1), 1995, pp. 185-186
Citations number
7
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
164
Issue
1
Year of publication
1995
Pages
185 - 186
Database
ISI
SICI code
0378-1119(1995)164:1<185:COARCE>2.0.ZU;2-9
Abstract
A 1.2-kb DNA fragment from Bacillus subtilis CD4 encoding endo-beta-1, 4-glucanase and cellobiase activities was cloned and expressed in Esch erichia coli. Carboxymethylcellulase (CMCase) and cellobiase activitie s were detected in a cell-free lysate of recombinant (re-) E. co ii (E c). The re-enzymes were functional in Ec, as it utilized carboxymethyl cellulose, soluble cellulose and cellobiose as sole carbon sources for growth.