TOXOPLASMA-GONDII IN-VITRO CULTIVATION - ECONOMIC AND EFFICIENT MASS-PRODUCTION

Based on a serum-free and CO2-independent culture of Toxoplasma gondii in vitro, a simple single arrangement mass production of trophozoites of the highly virulent BK strain of this parasite was developed. Star ting simultaneously, 10(7) host cells (HEp-2) and 10(7) Toxoplasma tro phozoites were cultured in one cell-factory(R) for 5 days, removing un attached cells, cell remnants and extracellular parasites by flushing daily. Each unit yielded about 0.5X10(9) parasites, which were almost free of any contamination with host cells and cell debris. As extensiv e manipulation of the cultures were avoided, a simple and reliable met hod for a mass production of Toxoplasma trophozoites has been develope d, distinguishing itself by a low medium consumption and a low work-ex penditure.