MOLECULAR-CLONING, SEQUENCING, CHROMOSOMAL LOCALIZATION, AND TISSUE DISTRIBUTION OF THE HUMAN NA+ H+ EXCHANGER (SLC9A2)/

The Na+/H+ exchangers belong to a family of transport proteins involve d in intracellular pH regulation and vectorial sodium transport across various epithelial tissues. We have cloned a unique isoform (NHE-2) b y screening a rat intestinal cDNA library utilizing a human NHE-1 frag ment. In this report we describe molecular cloning of the human Na+/H exchanger (NHE-2, HGMW-approved symbol SLC9A2). The cDNA encodes a pr otein of 698 amino acid residues. Human NHE-2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands. Hydropathy plot indicates that the translated pro tein is predicted to have 10 transmembrane domains with three potentia l N-glycosylation sites and four potential cAMP- and cGMP-dependent pr otein kinase phosphorylation sites. This cDNA maps the location of the gene encoding NHE-2 to human chromosome 2q11.2. (C) 1995 Academic Pre ss, Inc.