AN INTEGRATED YAC CLONE CONTIG FOR THE WAGR REGION ON HUMAN-CHROMOSOME 11P13-P14.1

The WAGR syndrome (Wilms tumor, aniridia, genito-urinary anomalies, an d mental retardation) deletion region on chromosome 11p13 has been ext ensively characterized by deletion analysis and long-range restriction mapping. A dense probe set is available for this genomic region, whic h harbors a number of disease gene loci, some of which still are not c loned The identification of candidates for these genes would be greatl y facilitated by a complete gene map for this chromosomal segment. As an initial step toward this goal, we have isolated the entire region i n 58 overlapping YAC clones. The contig spanning 8 Rib from RAG1 to KC NA4 has been assembled by STS and probe content mapping for 76 loci wi th an average spacing of about 100 kb, A subset of clones has been ana lyzed by PFG analysis to position these within the known physical map, Common microsatellite markers permit an alignment of the YAC contig w ith the genetic and radiation hybrid maps of chromosome 11. Ten known genes, some with much more refined map positions, are placed in the co ntig. The severalfold coverage of 11p13-p14.1 provides a reliable reso urce for the future development of a complete gene map of this region. (C) 1995 Academic Press, Inc.