CDNA SEQUENCE AND CHROMOSOMAL LOCALIZATION OF A NOVEL HUMAN PROTEIN, RBQ-1 (RBBP6), THAT BINDS TO THE RETINOBLASTOMA GENE-PRODUCT

We have previously isolated cDNA of a novel protein (RBQ-1, HGMW-appro ved symbol RBBP6) that binds to the retinoblastoma gene product (pRB). Total nucleotide sequence of the cDNA has now been determined. It enc oded a protein of 140 kDa that consists of 948 amino acids and contain s multiple repeated sequences like SRS, YRE, and VPPP. The region used for pRB binding was identified on a small region near the C-terminus. We have mapped this gene to 16p11.2-p12 using polymerase chain reacti on analysis on a human-hamster hybrid cell panel and chromosomal fluor escence in situ hybridization. (C) 1995 Academic Press, Inc.