LOCALIZATION AND PHYSICAL MAPPING OF THE PROSTATE-SPECIFIC MEMBRANE ANTIGEN (PSM) GENE TO HUMAN-CHROMOSOME-11

The prostate-specific membrane antigen (PSM) was identified by the mon oclonal antibody 7E11-C5.3, which was raised against the human prostat ic carcinoma cell line LNCaP (3). The PSM antigen is expressed by norm al, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA enc oding the 100-kDa PSM glycoprotein was cloned from LNCaP cells (4). St udies have shown that the expression of PSM is tissue-specific (5). In the present study monochromosomal somatic cell hybrids were used to l ocalize the PSM gene to human chromosome 11. Using this information, i nitial mapping studies identified two potential PSM gene loci at 11p11 .1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene. (C) 1995 Academic Press, Inc.