CHROMOSOMAL MAPPING OF THE MOUSE A3 ADENOSINE RECEPTOR GENE, ADORA3

A3 adenosine receptor is a member of Gi protein-coupled receptors that mediate inhibition of adenylate cyclase activity upon binding to the ligand. We determined the chromosome localization of the mouse A3 gene for future genetic studies, utilizing an interspecific backcross pane l formed from the cross (C57BL/6J X Mus spretus)F-1 X M. spretus. Geno mic DNAs from 94 individuals in the backcross were analyzed by Souther n hybridization with murine A3 receptor cDNA probe. Unique map positio ns were determined by haplotype analysis with 1388 previously mapped l oci in the mouse backcross. The mouse A3 receptor gene (Adora3) mapped to chromosome 3, in tight linkage with DNA marker D3Bir15. (C) 1995 A cademic Press, Inc.