DISRUPTION OF PML-ASSOCIATED NUCLEAR-BODIES DURING HUMAN CYTOMEGALOVIRUS-INFECTION

PML is a protein associated with discrete spherical structures within the nucleus of normal cells. A defect in PML expression is observed in acute promyelocytic leukaemia as a consequence of a chromosomal trans location involving the genes encoding PML and the alpha retinoic acid receptor (RAR alpha). Disruption of PML bodies also occurs during herp es simplex virus infection after the immediate early protein Vmw110 ha s become associated with PML bodies. In this study, we followed the fa te of PML bodies in human fibroblasts during the course of a human cyt omegalovirus (CMV) infection. Disruption of PML bodies was observed to be dependent on de novo CMV gene expression and to occur within 4 h p ost-infection, concomitant with the onset of CMV IE gene expression. A lthough a transient increase in the number of PML bodies could be obse rved in some cells, PML exists predominantly as a diffuse nuclear prot ein during both the early and fate stages of CMV infection. Although t he function of PML bodies is still uncertain, their disruption may be important for efficient herpes virus replication.