THE ACTION OF HYPOCHLOROUS ACID ON PHOSPHATIDYLCHOLINE LIPOSOMES IN DEPENDENCE ON THE CONTENT OF DOUBLE-BONDS - STOICHIOMETRY AND NMR ANALYSIS

Kinetics of the consumption of hypochlorous acid in its reaction with double bonds of unsaturated phospholipids and fatty acids were measure d using luminol chemiluminescence. Stoichiometry ratios between the co nsumption of HOCl/OCl- and the loss of double bonds vary from 2:1 to 1 :1. Highest values were found in DMPC liposomes containing 5 mol% olei c acid or OPPC. With increasing content of double bonds or higher numb ers of double bonds in a fatty acid acyl chain due to incorporated uns aturated fatty acids or phospholipids in DMPC liposomes the stoichiome try ratio falls continuously to 1:1. A ratio of about 1:1 was observed in multilamellar and unilamellar liposomes composed of egg yolk phosp hatidylcholine. Products of the reaction of oleic acid with hypochloro us acid were analysed by H-1-NMR spectroscopy. Chlorohydrins were form ed in both DMPC liposomes containing 5 or 40 mol% oleic acid.