MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY USING POLYMERIC HOLLOW FIBERS

In this paper, polymeric hollow fibers prepared from pH-stable polypro pylene were used as columns for micellar electrokinetic capillary chro matography (MECC). The electroosmotic flow (EOF) for polypropylene hol low fibers was evaluated in the pH range of 5.0-12.0. With untreated p olypropylene hollow fibers a stabilized but enhanced EOF was achieved when SDS was used in the buffer, decreasing the separation window for uncharged substances in MECC to impractical levels. Uncharged acrylami de and charged 2-acryloylamido-2-methylpropane sulfonic acid surface m odifications were used to lower the strength of the EOF, increase the separation window and prevent local overheating that could melt the co lumn wall.