BONE-MARROW INTERFACE - PREFERENTIAL ATTACHMENT OF AN OSTEOBLASTIC MARROW STROMAL CELL LINE

In this study, we report on the cell adhesion properties of marrow str omal cells to extracellular matrix components such as collagen and non collagenous proteins. The osteoblastic cells and their non-osteoblasti c counterparts (MBA series) from the marrow stroma differentially reco gnized a spectrum of extracellular matrix proteins. The osteoblastic c ells, MBA-15, preferentially attached to bone matrix proteins, whereas fibroendothelial MBA-2.1 and adipocytic 14F1.1 cells did not. The MBA -15 cells demonstrated a preference in their attachment to fibronectin > mixture of collagens > bone matrix extracts > collagen type I > non collagenous proteins. Clonal subpopulations derived from the MBA-15 ce ll line representing various stages along the osteogenic lineage expre ssed differential attachment preference. MBA-15.4, a less differentiat ed clonal line, was compared to MBA-15.6, a mature cell line. (C) 1995 Wiley-Liss, Inc.