HOMOLOGOUS CONDITIONED MEDIUM ENHANCES EXPRESSION OF TRH IN HYPOTHALAMIC NEURONS IN PRIMARY CULTURE

Primary cultures of hypothalamic cells maintained in the presence of s erum were either kept with homologous conditioned medium (CM) (i.e. on ly half of the medium was removed at each medium change) or without (t otal medium change). In cultures with homologous CM, TRH levels were i ncreased. The effects of CMs from various intervals of the primary cul ture were tested. The strongest increases of TRH levels were obtained with CM from cultures enriched with hypothalamic glia.