CLONING AND NUCLEOTIDE SEQUENCE OF THE INULIN FRUCTOTRANSFERASE (DFA-I-PRODUCING) GENE OF ARTHROBACTER-GLOBIFORMIS S14-3

A gene encoding an inulin fructotransferase (DFA I-producing) [EC 2.4. 1.200] from Al Arthrobacter globiformis S14-3 was cloned and the nucle otides sequenced, for the first time, The sequence indicated that the native enzyme protein is composed of 392 amino acid residues. The nati ve enzyme is an extracellar enzyme produced in the culture supernatant of A. globiformis S14-3, but the nucleotide sequence of the gene lack s a sequence for signal peptide for secretion. The 1.5-kb DNA fragment encoding the gene was found to produce the active enzyme in the cultu re supernatant of an E. coli clone, under the control of the lac promo ter of pUC119.