KINETIC-ANALYSIS OF AN AUTOCATALYTIC PROCESS COUPLED TO A REVERSIBLE INHIBITION - THE INHIBITION OF THE SYSTEM TRYPSINOGEN-TRYPSIN BY P-AMINOBENZAMIDINE

Citation
Mc. Manjabacas et al., KINETIC-ANALYSIS OF AN AUTOCATALYTIC PROCESS COUPLED TO A REVERSIBLE INHIBITION - THE INHIBITION OF THE SYSTEM TRYPSINOGEN-TRYPSIN BY P-AMINOBENZAMIDINE, Biological chemistry Hoppe-Seyler, 376(9), 1995, pp. 577-580
Citations number
18
Categorie Soggetti
Biology
ISSN journal
01773593
Volume
376
Issue
9
Year of publication
1995
Pages
577 - 580
Database
ISI
SICI code
0177-3593(1995)376:9<577:KOAAPC>2.0.ZU;2-Q
Abstract
A kinetic analysis of the mechanism of autocatalytic activation in the presence of a reversible inhibitor is presented. The kinetic equation s of both the transient phase and the steady state are derived for thi s mechanism. We have extended the kinetic equations derived to a parti cular case in rapid equilibrium conditions. This analysis is illustrat ed by the experimental study of the inhibition by p-aminobenzamidine o f trypsin activity in its action on trypsinogen. In such system, the a mount of active enzyme increases exponentially, as expected from an au tocatalytic process. The results obtained show that the apparent activ ation rate constant decreases non-linearly with the initial concentrat ion of inhibitor, according to the equations obtained in the kinetic a nalysis.