ADDITIONAL BIOACTIVE ACETOGENINS, ANNOMUTACIN AND (2,4-TRANS)-10R-ANNONACIN-A-ONES AND (2,4-CIS)-10R-ANNONACIN-A-ONES, FROM THE LEAVES OF ANNONA-MURICATA

In a continuation of our research on bioactive components from the lea ves of Annona muricata, three novel monotetrahydrofuran Annonaceous ac etogenins, namely, annomutacin [1], (2,4-trans)-10R-annonacin-A-one [2 ], and (2,4-cis)-10R-annonacin-A-one [3], have been identified. Their structures were deduced by ms, nmr, ir, and uv spectral and chemical m ethods, and the absolute configurations were determined by Mosher este r methodology. A known bioactive amide, N-p-coumaroyl tyramine, was al so found. Compound 1 and the mixture of compounds 2 and 3 showed selec tive cytotoxicities against the human A-549 lung tumor cell line.