A SPECTROSCOPIC APPROACH TO LIPOPROTEIN SUBCLASS ANALYSIS

Citation
J. Otvos et al., A SPECTROSCOPIC APPROACH TO LIPOPROTEIN SUBCLASS ANALYSIS, Journal of clinical ligand assay, 19(3), 1996, pp. 184-189
Citations number
24
Categorie Soggetti
Immunology
ISSN journal
10811672
Volume
19
Issue
3
Year of publication
1996
Pages
184 - 189
Database
ISI
SICI code
1081-1672(1996)19:3<184:ASATLS>2.0.ZU;2-X
Abstract
The particle subclasses of different size that comprise the major dens ity classes of plasma lipoproteins (VLDL, LDL, HDL) do not all exhibit the same associations with coronary artery disease (CAD). Thus, indiv iduals with comparable levels of LDL and HDL may be at very different risk of CAD owing to underlying differences in subclass distribution, All current methods to separate and quantify lipoprotein subclasses ar e time-consuming and labor-intensive, and, therefore, unsuitable for l arge clinical research studies or routine assessment of CAD risk in th e general population, We describe here a spectroscopic approach to lip oprotein subclass quantification that is rapid and efficient, allowing the simultaneous, automated quantification of 6 VLDL, 4 LDL, and 5 HD L subclasses in under 1 minute, The process, which employs proton nucl ear magnetic resonance (NMR) spectroscopy, exploits knowledge of the n atural particle size-dependent spectral differences that exist among t he subclasses, By avoiding the need for any physical separation steps or reagents, high throughput rates are possible and most sources of an alytical error are eliminated, This combination, plus the large inform ation content of each NMR LipoProfile, makes the NMR method an attract ive, cost-effective alternative to existing clinical means of assessin g and managing CAD risk.