THE EFFECT OF C5A AND U46619 ON THE ISOLATED, PERFUSED HUMAN PLACENTAL LOBULE - DEVELOPMENT OF A METHOD FOR THE ONLINE ESTIMATION OF TISSUE-FLUID ACCUMULATION
Am. Finch et al., THE EFFECT OF C5A AND U46619 ON THE ISOLATED, PERFUSED HUMAN PLACENTAL LOBULE - DEVELOPMENT OF A METHOD FOR THE ONLINE ESTIMATION OF TISSUE-FLUID ACCUMULATION, Journal of pharmacological and toxicological methods, 34(3), 1995, pp. 133-141
A method for the automatic and simultaneous determination of perfusion
pressure and fluid accumulation in the isolated, perfused human place
ntal lobule is described. We demonstrated that the inflammatory mediat
or, C5a, a C5a agonist analogue peptide, and the thromboxane mimetic U
46619 caused increased fetal perfusion pressure and increased tissue w
eight when administered via the fetal arterial circulation. Occlusion
of the fetal venous effluent tubing caused significantly greater incre
ases in tissue weight than the pharmacological agents. Detectable incr
eases in tissue weight occurred within 47 +/- 3 sec (n = 21) following
pressure increases caused by the pharmacological agents. In each case
, the increase in tissue weight was accompanied by an increased permea
bility of the materno-fetal barrier, shown by the transfer of Evans bl
ue albumin from the fetal circulation to the maternal compartment.