EPITOPIC ANALYSIS AND QUANTIFICATION OF BOVINE MYOGLOBIN WITH MONOCLONAL-ANTIBODIES

Seven rat monoclonal antibodies (MAb) for bovine myoglobin were produc ed. Five antibodies reacted with surface-adsorbed myoglobin whereas th e two remainders reacted only in a sandwich type ELISA. The ability of different antibodies to bind simultaneously to myoglobin was examined by competition and additivity experiments and three noncompeting epit ope regions were found, A two-site enzyme immunoassay was developed an d allowed quantification of 30 ng/ml bovine myoglobin. These antibodie s should be valuable tools in comparative studies for immunological re activity of mammalian myoglobins and for myoglobin measurement in seru m and urine of myopathic animals.