M. Fejtl et Do. Carpenter, NEURITE OUTGROWTH IS ENHANCED BY CONDITIONING FACTOR(S) RELEASED FROMCENTRAL GANGLIA OF APLYSIA-CALIFORNICA, Neuroscience letters, 199(1), 1995, pp. 33-36
Single neurons of Aplysia californica were cultured on control and con
ditioned medium-treated cover slips (CM, derived from central ganglia
of Aplysia) and fed with L-15 medium plus 20% hemolymph. After 5 h 36.
9 +/- 3.3% of all neurons plated on CM-treated cover slips sprouted, w
hereas only 22.9 +/- 2.7 % sprouted in the control group. After 24 h t
he average neurite length in the presence of putative conditioning fac
tor(s) was 621 +/- 42 mu m versus 411 +/- 20 mu m in control, but the
number of primary processes was the same (4.3 +/- 0.5 versus 4.2 +/- 0
.9).