New arrays of microtubules in the fission yeast Schizosaccharomyces po
mbe, which distribute in the cell in a cell cycle-dependent manner. we
re characterized using conventional and confocal laser scanning immuno
fluorescence microscopy. During the interphase and prophase, we observ
ed abundant cytoplasmic microtubules between cell poles, a peripheral
network of randomly and helically distributed cortical microtubules, a
nd perinuclear microtubules surrounding the nucleus. At the anaphase a
nd telophase, an equatorial ring containing tubulin was visualized. Th
is ring colocalized with an actin contractile ring, suggesting that th
ey may control the plane of cell division cooperatively.