8-METHOXYPSORALEN PHOTOADDUCT FORMATION IN COMPLEMENTARY OLIGONUCLEOTIDES CONTAINING A CROSS-LINKABLE SITE

Authors
PARK J AMICI L GASPARRO FP
Citation
J. Park et al., 8-METHOXYPSORALEN PHOTOADDUCT FORMATION IN COMPLEMENTARY OLIGONUCLEOTIDES CONTAINING A CROSS-LINKABLE SITE, Photodermatology, photoimmunology & photomedicine, 11(3), 1995, pp. 102-106
Citations number
NO
Categorie Soggetti
Dermatology & Venereal Diseases
Journal title
Photodermatology, photoimmunology & photomedicine → ACNP
ISSN journal
09054383
Volume
11
Issue
3
Year of publication
1995
Pages
102 - 106
Database
ISI
SICI code
0905-4383(1995)11:3<102:8PFICO>2.0.ZU;2-G
Abstract
The complete profile of 8-methoxypsoralen photoadduct formation in com plementary oligonucleotides (5'-GAGTATGAG and 5'-CATAC) has been deter mined. Equimolar solutions of the oligonucleotides were irradiated at 4 degrees C in order to stabilize the mini-double helix. Photomodified oligonucleotides were separated by reversed phase chromatography on a Vydac C4 column. Photoadduct formation favored the 5'TAT site in the 9mer over the 5'ATA site in the 5mer by a factor of two. Split-dose st udies showed that the monoadducts formed on GAGTATGAG were preferencia lly converted to cross-links by an additional UVA exposure.