ELECTROGENERATED CHEMILUMINESCENT DETERMINATION OF PYRUVATE USING TRIS(2,2'-BIPYRIDINE)RUTHENIUM(II)

A novel method for the sensitive and selective determination of pyruva te is presented, using an electrogenerated chemiluminescence (ECL) rea ction of tris(2,2'-bipyridine)ruthenium(II) in the presence of cerium( III) nitrate, increasing the number and diversity of analytes currentl y detected by this ECL reaction, Various experimental conditions have been optimized, lending to a calibration over three-orders of magnitud e of concentration, with a detection limit of 3.1 x 10(-7) mol l(-1) ( 27 ppb), Simple methods have also been developed to suppress the inter ference effects of various compounds that are expected to be present i n potential samples from fermentation media.