OPTICAL TWEEZERS-BASED IMMUNOSENSOR DETECTS FEMTOMOLAR CONCENTRATIONSOF ANTIGENS

Authors
HELMERSON K KISHORE R PHILLIPS WD WEETALL HH
Citation
K. Helmerson et al., OPTICAL TWEEZERS-BASED IMMUNOSENSOR DETECTS FEMTOMOLAR CONCENTRATIONSOF ANTIGENS, Clinical chemistry, 43(2), 1997, pp. 379-383
Citations number
6
Categorie Soggetti
Medical Laboratory Technology
Journal title
Clinical chemistry → ACNP
ISSN journal
00099147
Volume
43
Issue
2
Year of publication
1997
Pages
379 - 383
Database
ISI
SICI code
0009-9147(1997)43:2<379:OTIDFC>2.0.ZU;2-Y
Abstract
We used optical tweezers (optical trapping technology) to measure the force required to separate antigen-antibody bonds. Under competitive-b inding conditions, we used the force determination to detect and measu re protein antigen concentrations as small as 1 fmol/L (10(-15) mol/L) .