ABSORPTION CYTOMETRIC DNA ANALYSIS OF SECTIONS OF GASTRIC CARCINOMAS AND A COMPARISON WITH CYTOFLUOROMETRY OF SINGLE-CELL SUSPENSIONS AND FLOW-CYTOMETRY

Measurements were made of cellular deoxyribonucleic acid (DNA) content in paraffin-embedded materials from 44 gastric carcinomas, using thre e different methods; static cytometric light absorbance technique usin g tissue sections (SCM-abs), static cytometric fluorescence technique using single-cell suspensions (SCM-flu), and flow cytometry (FCM). The DNA ploidy determined by SCM-abs was grouped into low or high ploidy according to dispersion on the DNA histogram, and the DNA ploidy deter mined by SCM-flu and FCM was grouped into diploidy or aneuploidy, acco rding to the peak DNA value. Accord between SCM-abs and SCM-flu or bet ween SCM-abs and FCM was confirmed when low ploidy by SCM-abs showed d iploidy by SCM-flu or FCM and high ploidy by SCM-abs showed aneuploidy by SCM-flu or FCM. An incomplete but good accord of DNA ploidy was ob tained with these methods. (C) 1995 Wiley-Liss, Inc.