EFFECT OF THE INHIBITOR OF NITRIC-OXIDE SYNTHASE, N-G-NITRO-L-ARGININE METHYL-ESTER, ON CEREBRAL AND MYOCARDIAL BLOOD FLOWS DURING HYPOXIA IN THE AWAKE DOG

The increase in cerebral blood flow (CBF) elicited by moderate hypoxia in anesthetized animals is little attenuated by nitric oxide (NO) syn thase inhibitors. However, in previous studies, the effects of NO synt hase inhibitors may have been altered by anesthetics. Consequently, we studied the effects of the NO synthase inhibitor, N-G-nitro-L-arginin e methyl ester (L-NAME), on cerebral and myocardial blood flows during hypoxia in the awake dog. Regional CBF and myocardial blood flows (MB F) were measured under normoxia and hypoxia in 16 beagle dogs after an intravenous (IV) injection of either saline (control, n = 8) or L-NAM E 20 mg/kg (n = 8). One week after thoracotomy for catheter insertion, awake dogs were studied during three periods: normoxia and after 2 an d 4 h of normocapnic hypoxia in an environmental chamber (Fro(2) = 0.1 0, FICO2 =: 0.035, balance N-2). At each stage, a bolus injection of L -NAME or saline was followed 15 min later by left atrial injection of radiolabeled microspheres (Ce-141, Ru-103, (SC)-S-46) for regional CBF and MBF. After the dogs were killed, the brain and the heart were fix ed in 10% formaldehyde, dissected by region and weighed, and radioacti vity was measured in a gamma counter. During hypoxia, Pao(2) was appro ximately 45 mm Hg with normal Paco(2). In the control group, CBF incre ased by 45% after 2 h a:nd 48% after 4 h of hypoxia; MBF increased by 69% and 60%, respectively. L-NAME prevented the CBF increase during hy poxia and the MBF increase after 2 h of hypoxia; after 4 h of hypoxia the measurement of MBF was confounded by cardiac dysfunction. These re sults suggest that NO plays a role in cerebral vasodilation during hyp oxia in the awake animal.