SEQUENCING OF A 40.5 KB FRAGMENT LOCATED ON THE LEFT ARM OF CHROMOSOME-VII FROM SACCHAROMYCES-CEREVISIAE

The nucleotide sequence of a 40.5 kb DNA fragment from the left arm of chromosome VII of Saccharomyces cerevisiae was determined and analyse d. Twenty-eight open reading frames (ORFs) longer than 300 nucleotides were identified. Eight of them correspond to the following known yeas t genes: EMP24, GCN1, SPO8, COX13, CDC55, RPS26, COX4 and LSR1, also c alled GTS1. Twelve ORFs are new, among them eight show homology with o ther genes while four have no homology with any sequence in the databa ses. Eight additional ORFs are internal to or partially overlapping wi th other ORFs. The nucleotide sequence reported here is deposited in t he EMBL database under the Accession Numbers X91837 and X91489.