INFLUENCE OF DIFFERENT LUMINOLS ON THE CHARACTERISTICS OF THE CHEMILUMINESCENCE REACTION IN HUMAN NEUTROPHILS

In search for a luminol with very high output of light, 20 different l uminol samples were tested for their ability to enhance the chemilumin escence reaction in phorbol myristate acetate activated human neutroph ils. We found that the majority of luminols tested (17 samples) gave a lmost the same light output from neutrophils, and that the major part of the activity was from an intracellular origin, Owing to the fact th at three isoluminol samples were unable to monitor respiratory burst a ctivity taking place intracellularly, a very low level of chemilumines cence was obtained with these samples, Their light output was, however , greatly increased when horseradish peroxidase or myeloperoxidase was added, showing that the light-generating reaction with isoluminol as well as with luminol is peroxidase-dependent. The fact that isoluminol could also use myeloperoxidase as amplifying peroxidase, suggests tha t the lack of measurable intracellular activity in the presence of iso luminol is somehow related to a limited or restricted diffusion of the molecule to intracellular sites, The isoluminol system constitutes a sensitive system for measuring release of oxygen metabolites from phag ocytic cells.