A METHOD FOR PURIFICATION OF CLOSTRIDIUM-PERFRINGENS PHOSPHOLIPASE-C FROM RECOMBINANT BACILLUS-SUBTILIS CELLS

Authors
HIRATA Y MINAMI J KOYAMA M MATSUSHITA O KATAYAMA SI JIN F MAETA H OKABE A
Citation
Y. Hirata et al., A METHOD FOR PURIFICATION OF CLOSTRIDIUM-PERFRINGENS PHOSPHOLIPASE-C FROM RECOMBINANT BACILLUS-SUBTILIS CELLS, Applied and environmental microbiology, 61(11), 1995, pp. 4114-4115
Citations number
15
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
11
Year of publication
1995
Pages
4114 - 4115
Database
ISI
SICI code
0099-2240(1995)61:11<4114:AMFPOC>2.0.ZU;2-J
Abstract
We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method c onsists of three purification steps, and it allowed us to obtain 6.2 m g of pure phospholipase C from 800 ml of culture.